Introduction into Molecular Methods in Cancer Diagnosis - Dr. Matthew Clarke

Explore molecular techniques used in cancer diagnosis across cellular pathology subspecialties in this comprehensive talk. Learn about immunohistochemistry, methylation profiling, fluorescence in situ hybridisation (FISH), and various sequencing modalities. Discover the advantages of each technique for pathologists in accurately characterizing tumors and identifying patients suitable for targeted therapies. Delve into topics such as tissue assessment, DNA mutations, DNA methylation in neuropathology, challenges in methylation profiling, DNA copy number interpretation, fusion translocations, and sequencing examples in sarcomas and brain tumors. Gain insights into improved diagnostic methods and their applications in different cancer types.

Introduction
Overview
Tissue assessment
DNA and mutations
Immunist chemistry
Summary
DNA Methylation
DNA Methylation in Neuropathology
Improved Diagnosis
Summary of methylation profiling
Challenges of methylation profiling
DNA copy number interpretation
Copy number plot
Copy number profile
Fusions translocations
Types of fusions
Definition of a fusion
Entrac fusions
Ntracks
Sequencing
Example
Sarcoma
Brain tumors
Fluorescence in situ hybridization
PCR