Introduction to microscopy

This course covers the learning outcomes of understanding the principles of microscopy, including the light microscope, additional systems on light microscopes, histochemical techniques, immunohistochemistry (IHC), interpreting the appearance of sections, and fluorescence microscopes. Students will learn how to use a light microscope effectively, apply phase contrast and filters, interpret sections, and understand the Z-plane. The teaching method includes theoretical explanations and practical demonstrations. This course is intended for individuals interested in gaining foundational knowledge in microscopy.

• Introduction
• Learning outcomes
• 1 Microscopy
• 1 Microscopy
• 1.1 The light microscope
• 1.2 Using a light microscope
• 2 Additional systems on light microscopes
• 2 Additional systems on light microscopes
• 2.1 Phase contrast
• 2.2 Filters
• 2.3 Grids and graticules
• 3 Histochemical techniques
• 3 Histochemical techniques
• 4 Immunohistochemistry (IHC)
• 4 Immunohistochemistry (IHC)
• 5 Interpreting the appearance of sections
• 5 Interpreting the appearance of sections
• 5.1 The Z-plane
• 6 Fluorescence microscopes
• 6 Fluorescence microscopes
• 6.1 The fluorescence confocal microscope
• Conclusion
• Acknowledgements