Advanced Microbiology

The Advanced Microbiology is a 33 module course on advanced microbiology, which will equip the students with certain advanced aspects of basic microbiological techniques that include detailed classification of viruses, yeast and fungi. Production of antibiotics and their effect on the normal and pathogenic microflora of human body. Certain beneficial aspects of soil bacteria in increasing the soil fertility are also considered here. The most attractive feature of this module is that there are 15 modules of practical microbiology, which include preparation of culture media for different microbes, handling of different organisms, sterilization techniques, and isolation of microbes from nature, biomass measurement, and antibiotic sensitivity of microbes. Successful completion of this module will enable a student to perform practical hands on basic microbiological techniques that can be quite handy whenever he or she joins any research laboratory or even in industry.The 33 modules of this course will be delivered over a period of 9 weeks roughly covering the working days of a semester. For the first 7 weeks, 4 modules will be uploaded and a time slot will be reserved for live interaction with the students. During the last 2 weeks, 3 + 2 modules will be uploaded.

Week1 Module 1:Morphology, Disease and Holmes Classification. Module 2:Classification of viruses-I Module 3:Classification of viruses II   Week 2 Module 4: Classification of fungi: Classical Concept -I Module 5: Classification of fungi: Classical Concept -II Module 6: Modern Classification of fungi: Modern Concept - I

Week 3 Module 7:Classification of fungi: Modern Concept - II Module 8:Classification of fungi: Modern Concept - III
Module 9:Classification of yeast-I   Week 4 Module 10:Classification of yeast-II Module 11: Application of microbes Module 12:Antibiotics: The secondary Metabolites   Week 5 Module 13:Role of Staphylococcus in infection Module 14:Nitrogen fixation & Role of Nif and Nod genes. Module15:Microbiology & Human Health

Week 6 Module 16: Bacterial Diseases Module 17.The pathogenesis of Vibrio cholerae Module 18: Introduction to Salmonella

Week 7 Module 19: Preparation of Culture Media: Liquid Medium Module 20: Preparation of Culture Media: Solid Medium
Module 21: Sterilization Techniques (Practical)   Week 8
Module 22: Isolation of single colony on solid media (Practical) Module 23:Enumeration of bacterial numbers by serial dilution & plating (Practical) Module 24:Measurement of fungal bio-mass   Week 9 Module 25: Animal Handling (Practical) Module 26:Staphylococcus aureus Module 27:Isolation of protozoa from soil

Week 10 Module 28:Antibiotic sensitivity assay: natural products Module 29: Preparation of culture media for pathogenic bacteria – part 1 Module 30: Preparation of culture media for pathogenic bacteria – part 2   Week 11 Module 31:Albert and Giemsa Staining Module 32:Determination of Growth Phase of E. coli by Measurement of OD & cfu

Week 12 Module 33:Preparation of Buffer Solution