CRISPR/Cas-Enabled Paper Microfluidics Device for Rapid Diagnosis

Explore a cutting-edge webinar on CRISPR/Cas-enabled paper microfluidics for rapid pathogen diagnosis. Discover how Dr. Zhugen Yang, a faculty member at Cranfield University, presents a low-cost, deployable paper-based biosensor device for quick analysis of pathogens, applicable to infectious disease diagnosis and wastewater surveillance. Learn about the innovative wax-printed paper microfluidic device with hydrophobic and hydrophilic layers, enabling sample processing through folding and unfolding. Delve into topics such as the AGATHA sensor for viral RNA detection, wastewater-based epidemiology as a tool for predicting pandemics, and on-site nucleic acid enrichment methods. Gain insights into the detection mechanism using CRISPR/CAS-RT-LAMP and the potential of paper-origami devices in public health applications. This 26-minute presentation offers a comprehensive look at the future of rapid, accessible pathogen detection and its implications for global health monitoring.

Intro
с Challenges: pathogen detection
Outline & Learning Objectives
Our approach: how does paper-origami device work?
Design and mechanism of new AGATHA sensor for viral RNA
Wastewater surveillance as a new public health tool
Wastewater-based epidemiology - a potential solution for predicting next pandemic?
What's going on wastewater surveillance worldwide?
A on-site nucleic acid enrichment method: Move sewage testing from the lab to the field
Detection mechanism with CRISPR/CAS-RT-LAMP
Paper-Origami Devices for Diagnosis and Sensing Wastewater for Public Health
Our contribution on wastewater surveillance for early warning of pandemic