rRNA Sequencing Analysis and Visualization

Explore microbiome analysis techniques and visualization tools in this 44-minute video tutorial. Learn about 16s rRNA sequencing analysis, including OTU identification, clustering, phylotype analysis, and diversity measurements. Discover how to perform principal component analysis (PCA) and AMOVA between treatments using R. Master tree plotting with ggtree and its customization options. Gain insights into using Galaxy with Krona for interactive visualizations and MEGAN6 for comparative analysis. Follow along as the tutorial covers input file formats, analysis goals, and practical applications of various bioinformatics tools, providing a comprehensive overview of microbiome data analysis and visualization techniques.

Introduction
Inputs required
Analyses Included in this video
Goals for this video
Looking at the input files format
OTU identification and clustering
Phylotype Analysis
Alpha and Beta Diversity
PCA output in R
AMOVA between treatments
ggtree for tree plotting in R
Customization of ggtree
Galaxy + Krona
MEGAN6
Closing summary