Genetic Engineering and Its Recent Advancements

In today’s world, genetic engineering plays an important role in drug discovery and pharmaceutical industries. Various hormones, proteins, interferons, antibodies and other therapeutics can be produced by introducing rDNA/foreign gene to the host organism. Genetic engineering can also be used for controlling various environmental factors like air, water, and soil pollution. It also plays a major role in waste management and bio remediation. Using this technique, the resistant varieties of crops can be developed with yield enhancement. After successful completion of the course, students will be able to learn the basic concepts of genetic engineering, its tools and techniques, cloning, applications of cloning, recent advancements and about transgenic plants and animals. The students will be able to think critically and apply their knowledge in conducting research projects.

Week

Module

Titles

Week 1

Module I: Fundamentals of Genetic Engineering

Introduction to Genetic Engineering

Tools of Genetic Engineering

Steps involved in Genetic Engineering

Week 2

Module II: Cloning, Types of Vectors and Genetic Libraries

Principles of cloning

Applications of cloning

Week 3

Different types of libraries

Library Screening

Week 4

Cloning and Expression vectors

DNA replication

Rolling circle model

Theta replication model

Bacterial Artificial Chromosome, Yeast Artificial Chromosome

Mammalian Artificial Chromosome

Week 5

Module III: Introduction of DNA into host

Genetic Manipulation of cells

Types of Transfection

Genetic manipulation in plants

Transgenic Plants

Transgenic Animals

Week 6

Module IV:

Gene silencing and regulation of Gene expression

Gene silencing

m RNA, si RNA, RNAi

Knock out technology

Week 7

Gene Expression

Regulation of Gene Expression in prokaryotes

Transcription and its regulation in prokaryotes

Translation and its regulation in prokaryotes

Week 8

Module V: Restriction modification system and Applications of Genetic Engineering

Restriction Endonucleases and its Types

Restriction modification system

Reporter genes

Microarrays

DNA foot printing

Week 9

Gene Therapy

Future trends in transgenic plants

Future trends in transgenic animals

Pharm animals

Week 10

Module VI: Genome Editing

Genome editing

CRISPR-Cas technology

Week 11

Mechanism of CRISPR-Cas

Case studies of CRISPR-Cas

Week 12

Basic instrumentations in Genetic Engineering

Sequence analysis