Real Time Tracking of Root Hair Nucleus Morphodynamics Using a Microfluidic Approach

Watch a 28-minute webinar from the TPJ series exploring real-time tracking of root hair nucleus morphodynamics using microfluidic techniques. Delve into Marie-Edith Chabouté's research on nuclear mechanics and dynamics in plant development, focusing on Arabidopsis root hair analysis. Learn about the importance of the plant nucleo-cytoplasmic interphase, nuclear shape changes, and the role of the nucleus as a rheostat in plant development. Discover the use of coverslip-based microfluidic devices for studying nuclear dynamics during tip growth and root hair development. Gain insights into nuclear mechano-transduction in plant development and explore potential future applications of microfluidic approaches in this field.

Intro
Nuclear mechanics and dynamics in plant development
Nuclear shape changes in plant development
Mechanical stress reaching the nucleus?
Importance of the plant nucleo-cytoplasmic interphase
Change in nuclear shape starts beyond a threshold
Morphodynamics and mechanics of nucleus are reversible
Nucleus as a rheostat in plant development
Root hair (RH): a perfect model to study nuclear dynamics
Coverslip based microfluidic device (CMD)
Arabidopsis RH analysis: a microfluidic approach
Experimental procedure
Nuclear dynamics during tip growth
Nuclear dynamics: changes in nuclear shape during tips
Nuclear dynamics during RH development
First conclusions
Further exploration of microfluidic approaches
Nuclear mechano-transduction in plant development
Acknowledgements