Towards a Computational Model of the Brain - Tools for Mapping and Perturbing Neural Circuits - Ed Boyden

Explore a comprehensive lecture on developing computational models of the brain and advanced tools for mapping and manipulating neural circuits. Delve into the structure of neurons, action potentials, and the unknowns in neuroscience. Learn about innovative techniques such as polymer mesh expansion, superresolution microscopy, and lightsheet microscopy for scalable brain imaging. Discover protocols for expansion microscopy and its applications in visualizing microtubules and synapses. Gain insights into cutting-edge approaches for quantitative analysis of neural structures, including the fruit fly brain and Brainbow technology. Understand the potential impact of these tools on unraveling the complexities of brain function and advancing our understanding of neuroscience.

Intro
Structure of Neurons
Action Potentials
Schematic Cartoon
The Unknowns in Neuroscience
Glia
Overview
Brain Tissue
Terry Tanaka
Peter Harris Christine Dryer
Summary
Polymer mesh
Softening the specimen
Expanding the specimen
Timelapse
Analysis
Expanding a biological object
Superresolution microscopy
Lightsheet microscopy
Lattice lightsheet microscopy
Scalable imaging of brains
Quantitative analysis
Fruit fly brain
No more science
Brainbow
Protocols
Expansion
Microtubules
synapses
unknowns
expansion microscopy