Tips and Tricks for Successful Multiplexing IHC
Offered By: Labroots via YouTube
Course Description
Overview
Syllabus
Intro
Multiplexed immunohistochemistry (mIHC)
Optimization of sample preparation
Blocking autofluorescence
Tips for blocking background
How to plan a successful multiplexed IHC experiment
How to choose an appropriate primary antibody?
The Thermo Fisher Scientific antibody search tool
Primary antibody protocol optimization
How do you choose an appropriate secondary antibody?
Secondary antibody protocol optimization
Types of labeling strategies
How to decide appropriate labeling strategy?
Direct conjugates of primary antibody
Standard secondary antibody labeling
Avidin-biotin amplification
Enzyme amplification
Points to remember for labeling strategy
Multiplex fluorescence: The main colors and beyond...
How to choose the optimal fluorophore and filter?
Spectral overlap and multiplexing
Fluorophore photobleaching and photostability
Controls and multiplexing
Some important rules to remember for multiplexed IHC
Taught by
Labroots
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