Synthetic Biology and Materials Science - Nature-Made Plastics

Explore the fascinating world of synthetic biology and materials science in this informative video tutorial. Delve into the process of engineering microorganisms to produce Tulipalin A, a compound used in manufacturing plastics without relying on crude oil. Learn about the groundbreaking work being done at biotech firm Arzeda, including the challenges and intricacies of this innovative approach. Discover how enzymes are programmed into microorganisms to catalyze a series of reactions, producing desired compounds. Gain insights into the advantages of PMMA (polymethyl methacrylate) and the importance of developing sustainable alternatives to petroleum-based plastics. Follow along as the video breaks down the steps involved in creating nature-made plastics, from selecting enzymes to optimizing and testing them. Understand the potential impact of this technology on reducing dependence on fossil fuels and creating more environmentally friendly materials.

Intro
we can select a series of enzymes from a catalog that will produce some desired industrial compound
petroleum/crude oil
methyl methacrylate (MMA)
advantages of PMMA
production of PMMA from crude oil is not sustainable
oil in the ground will eventually run out
Project Lead: Aaron Korkegian
a-methylene-y-butyrolactone (MBL)
fermentation host (yeast, bacteria, etc.)
enzyme database + efficient algorithm
natural substrate
the methyl may clash in the active site
Proposed Pathway
Generating the Enzymes
Testing the Enzymes
Optimizing the Enzymes
Testing the Optimized Enzymes
Confirmed Pathway
we can modify the ribosomal binding site sequences for any of the enzymes
any such reaction can be run in precisely the same large vat with the same material
PROFESSOR DAVE EXPLAINS