Mechano-Epigenetics for Cell Engineering: Biophysical Regulation and Applications

Explore mechano-epigenetics for cell engineering in this 43-minute lecture by Song Li, Chancellor's Professor and chair of UCLA's bioengineering department. Delve into Li's interdisciplinary research on mechanotransduction, cell engineering, and regenerative medicine. Learn about biophysical regulation of epigenetic states, cell differentiation, and reprogramming. Discover how nuclear shape influences histone modifications and how intracellular tension reduction enhances reprogramming efficiency. Examine the effects of mechanical loading on cells and nuclei using a single-cell deformation microfluidic platform. Investigate how nucleus deformation impacts neuronal gene activation, chromatin accessibility, and nuclear lamina structure. Compare biophysical and chemical effects on different cell types and gain insights into the field of mechano-epigenetics for advancing cell engineering techniques.

Symposium on Physical Genomics April 21, 2023
Cell and Tissue Engineering
Biophysical Regulation of Epigenetic State?
Mechanotransduction to Epigenetic Modulation
Cell Differentiation and Reprogramming
Nuclear Shape Regulates AcH3 and H3K4me2/3
Reduction of Intracellular Tension Enhances Reprogramming Efficiency
Synergistic Effects of Tension Reduction and Reprogramming Factors
Increases Chromatin Opening
Mechanical Loading on Cell and Nucleus
Single Cell Deformation Microfluidic Platform
Squeezing Decreased Cell Stiffness
Nucleus Deformation Enhanced Neuronal Gene Activation
Nucleus Deformation Decreased H3K9me3
Nuclear Deformation Increases Chromatin Accessibility at the Promoter of Neuronal Genes
Biophysical vs Chemical Effects
Squeezing Caused Partial Disassembly and Wrinkling of Nuclear Lamina
Squeezing Effects on Different Cell Types
Conclusions