Leveraging Evolutionary Trade-Offs and Phage Selection Pressure to Reduce Bacterial Pathogenicity

Explore the potential of phage therapy in combating bacterial pathogens through a comprehensive lecture on evolutionary trade-offs and selection pressure. Delve into the renewed approach of phage therapy, learning about isolating phage candidates, incorporating genome sequencing, and measuring phage host-range. Understand the characterization of phage binding and how trade-offs can benefit phage therapy. Discover methods to identify phages that drive antibiotic resistance and virulence trade-offs, and determine the likelihood of mutation-caused trade-offs. Examine a case study of emergency phage therapy in human volunteers, analyzing clinical samples before and after treatment. Gain insights into evolutionary predictions and their implications for future therapeutic approaches.

Intro
Leveraging evolutionary trade-offs and phage selection pressure to reduce bacterial pathogenicity
Phage therapy: a renewed approach
Isolating phage candidates
Incorporating genome sequencing
Measuring phage host-range
Characterizing phage binding
Trade-offs WOULD benefit phage therapy
Identify phages that drive antibiotic resistance trade-offs
Determine likelihood that mutations cause trade-offs
Identify phages that drive virulence trade-offs
Trade-ups WOULD NOT benefit phage therapy
Case 1: Emergency phage therapy in human volunteer
Examining clinical samples pre- vs. post-therapy
Evolutionary prediction