Automating High-throughput Luminex Assays for Multiplex Gene and Protein Analysis

Explore high-throughput Luminex assays for multiplex gene and protein analysis in this 45-minute panel presentation. Learn about Invitrogen ProcartaPlex and QuantiGene Plex assays, which offer a multi-omics approach using the Luminex platform. Discover how genomic and proteomic workflows can be combined without compromising data interpretation or sensitivity. Gain insights into investigating cell functions and responses by simultaneously interrogating large sets of RNA or protein targets in single samples using the 384-well format. Delve into topics such as high-throughput screening, xMAP bead-based assay technology, and automated workflows. Explore applications in 3D cell culture compound screening and biomarker studies of iPSC-derived cells. Understand the lab automation capabilities, including the inSPIRE Platform and Momentum workflow scheduling software, that enhance throughput and efficiency in these assays.

Intro
Thermo Fisher SCIENTIFIC
High-throughput screening A large-scale operation
High-throughput protein and gene expression assays
Luminex xMAP multiplex assays
Multiplex gene and protein expression profiling
Two applications-1 instrument in 384-well format
xMAP bead-based assay technology
High-throughput ProcartaPlex 384-well assays
ProcartaPlex Analysis App
High throughput multiplex gene expression analysis: QuantiGene Plex assay
High-throughput cell screening workflow using QuantiGene Plex 384-well assay
Summary of Workflow and Equipment
Application: 3D cell culture compound screen
Application: Biomarker studies of iPSC-derived cells
Data analysis workflow for QuantiGene Plex assays
Desired Throughput
About the Lab Automation team
Lab automation capabilities
Lab automation journeys
inSPIRE Platform
Automation of QuantiGene Plex Assay
Momentum workflow scheduling software
Increasing throughput with the Cytomat incubator