Next Generation Sequencing 2 - Illumina NGS Sample Preparation - Eric Chow

Explore the intricacies of Illumina Next Generation Sequencing (NGS) sample preparation in this 25-minute video lecture by Eric Chow from UCSF. Gain a comprehensive understanding of DNA and RNA library preparation techniques, learn about bead-based cleanup methods using Ampure or SPRI, and discover essential sample quantification and quality control procedures. Delve into the revolutionary advancements in DNA sequencing efficiency, comparing traditional Sanger sequencing to modern NGS methods. Understand the chemistry behind NGS, including how Illumina sequencers detect and display results using fluorescently labeled nucleotides. Examine alternative NGS technologies, their benefits, and limitations. Explore medical applications of NGS, such as non-invasive prenatal testing and cancer detection. Benefit from practical advice on purifying DNA samples with magnetic beads and assessing nucleic acid sample quality using an Agilent Bioanalyzer.

- Start
- Review of next generation sequencing
- DNA library preparation
- RNA library preparation
- Bead-based cleanups
- Sample quantification and quality control