Confocal Microscopy and Its Applications in Biomolecular Diffusion Studies - Lecture 1

Explore confocal microscopy and its applications in a 32-minute video presentation by Fatma Pri-Cakmak from MIT. Delve into the history of microscopes, modern microscopy techniques, and laser scanning confocal microscopy. Learn about the diffusion of biomolecules in vesicle-coated RNA-based coacervates and how cellular activities can be compartmentalized by phase separation. Discover the PolyU/spermine coacervate system, partitioning in coacervates, and concentration calculations using microscopy. Understand Fluorescence Recovery After Photobleach (FRAP) techniques for studying partial and entire droplet diffusion. Examine vesicle assembly around coacervate droplets and RNA diffusion in these structures. Part of the NACK - Nano-Educators Topical Seminar Series, this comprehensive overview provides valuable insights into advanced microscopy techniques and their applications in studying complex biological systems.

Confocal Microscopy
The Size of Things
History of Microscopes
History of Microscopes
History of Microscopes
History of Microscopes
Modern Microscope
Modern Microscopy
Laser Scanning Confocal Microscopy
Laser Scanning Confocal Microscopy
Laser Scanning Confocal Microscopy
Laser Scanning Confocal Microscopy
Diffusion of Biomolecules in Vesicle Coated RNA-Based Coacervates
Activities in cell can be compartmentalized by phase separation
Diffusion of biomolecules indicates dynamic structure
PolyU/spermine coacervate system
Partitioning in coacervate
Concentration in the droplet can be calculated using microscopy
Partitioning in PolyU/spermine coacervate system
Partitioning in PolyU/spermine coacervate system
Partial Diffusion - What is Fluorescence Recovery After Photobleach FRAP?
Entire Droplet Diffusion- FRAP
FRAP Curve and equations
Partial droplet bleaching RNA recovery in polyU/spermine coacervate
Partial droplet bleaching RNA recovery in polyU/spermine coacervate
Entire droplet bleaching RNA recovery in polyU/spermine coacervate
Entire droplet bleaching RNA recovery in polyU/spermine coacervate
Vesicle assembly around coacervate droplets
Vesicle assembly around coacervate droplets
RNA diffusion in vesicle coated coacervate droplets
Conclusions