Basics of Gene Editing and CRISPR - 2023 MPG Primer

Explore the fundamentals of gene editing and CRISPR technology in this 40-minute Medical and Population Genetics Primer video presented by Daniel Strebinger from the Broad Institute. Gain insights into the evolution of genome editing techniques, from zinc fingers to CRISPR, and understand their applications in human and mammalian cells. Delve into advanced concepts such as CRISPR nickases, base editing, and prime editing. Learn about the therapeutic potential of CRISPR, including its application in treating cell diseases and gene reactivation. This comprehensive overview, part of the Broad Institute's series on medical and population genetics, is designed for a wide audience ranging from research technicians to established investigators entering the field.

Introduction
What is genome editing
The problem with CRISPR
Zincfingers
Nonendogenous templates
Tail affectors
CRISPR
CRISPR C9
Domain architecture
Does it work in human cells
Does it work in mammalian cells
Where did CRISPR come from
CRISPR nickases
Base editing
Adenin base editing
Prime editing
CRISPR Therapeutic
C cell disease
Reactivation