Mechanism of Action of Erythromycin, Azithromycin, Clarithromycin, Chloramphenicol, and Clindamycin

Delve into a comprehensive lecture on the mechanisms of action of Erythromycin, Azithromycin, Clarithromycin, Chloramphenicol, and Clindamycin. Explore the similarities between these antibiotics as protein synthesis inhibitors, focusing on their interactions with bacterial ribosomes, specifically the 50S subunit and 23S ribosomal RNA. Understand the role of peptidyl transferase and how these antibiotics affect peptide chain elongation. Examine the specific mechanisms of each antibiotic, including their bacteriostatic and bactericidal properties. Compare the binding sites and effects of Clindamycin, Macrolides, and Chloramphenicol on bacterial protein synthesis. Gain insights into the post-antibiotic effects and potential impacts on toxin production and microbial opsonization.

Introduction and overview of lecture
Overview of similarities between Clindamycin, Macrolide and chloramphenicol
Clindamycin, macrolide Erythromycin and chloramphenicol work as protein synthesis inhibitors
MOA of clindamycin, macrolide Erythromycin and chloramphenicol on Bacterial Ribosomes
MOA of clindamycin, macrolide Erythromycin and chloramphenicol on larger 50s ribosomal subunit
Action of clindamycin , macrolide Erythromycin and Chloramphenicol on 23s Ribosomal RNA
Summary of above 4 similarities in the MOA of Clindamycin , Macrolide Erythromycin and Chloramphenicol
MOA of Clindamycin, Macrolide Erythromycin and Chloramphenicol on Ribozyme
What is Peptidyl Transferase?
Summary of all above similarities between Clindamycin, Macrolide Erythromycin and Chloramphenicol
Detailed explanation on the functions of Peptidyl Transferase
Transpeptidation and Ribosomal Translocation
Peptide chain elongation
How Clindamycin, Macrolide Erythromycin and chloramphenicol inhibit the protein synthesis by inhibiting the chain elongation process?
Summary of similarities between Clindamycin, Macrolide Erythromycin and Chloramphenicol
MOA; Clindamycin
MOA; Clindamycin as bacteriostatic and bactericidal
Summary of MOA of Clindamycin
MOA; Macrolides Erythromycin
MOA; Chloramphenicol binding site.
MOA; Comparison of binding site of clindamycin vs macrolide vs chloramphenicol.
MOA; Chloramphenicol.