Light & Single-Molecule Spectroscopy, Imaging, & Photocontrol

Explore the foundations of super-resolution microscopy in this illuminating lecture by 2014 Nobel Prize winner W.E. Moerner from Stanford University. Delivered at the APS March Meeting 2015 Kavli Foundation Special Symposium: Frontiers of Light, delve into the world of light and single-molecule spectroscopy, imaging, and photocontrol. Discover the journey from early steps in single-molecule spectroscopy to groundbreaking developments in super-resolution microscopy. Learn about statistical fine structure in inhomogeneously broadened lines, single-molecule imaging and tracking examples, and the surprises uncovered from isolated single molecules. Understand key concepts such as super-localization and active control of emitter concentration through sequential imaging. Gain insights into the detection of single-molecule absorption from emitted fluorescence at room temperature and the imaging of blinking and photorecovery for single EYFP. Explore applications in observing Huntingtin aggregates and the development of new fluorophores and 3D imaging methods. Appreciate the impact of single-molecule spectroscopy and imaging on various fields of science. This 36-minute talk offers a comprehensive overview of the revolutionary techniques that have transformed our ability to visualize the microscopic world with unprecedented clarity.

Light and Single-Molecule Spectroscopy, Imaging, and Photocontrol: Foundations for Super-Resolution Microscopy
Optical Spectroscopy of Molecules in Solids at Low T
Early Steps Toward Single Molecule Spectroscopy
Statistical Fine Structure in an Inhomogeneously Broadened Line
Single-Molecule Imaging and Spectroscopy
Some of the Surprises from Single Molecules!
Motivations and Impact: Single-Molecule Spectroscopy and Optical Imaging in Complex Systems
Detecting Single-Molecule Absorption from Emitted Fluorescence at Room T
Single-Molecule Imaging and Tracking Examples Much to Learn from Isolated Single Molecules!
1997: Imaging, Blinking, and Photorecovery for Single EYFP
Super-Resolution Microscopy Approaches not requiring single-molecule imaging
Key Idea #1: Super-Localization
Toward Super-Resolution with Single-Molecule Emitters
Key idea #2: Active control of emitter concentration, sequential imaging
What the data look like.... eYFP fusions to a bacterial protein blinking in
Observing Huntingtin Aggregates at
New Fluorophores, New 3D Imaging Methods
Impact of Single-Molecule Spectroscopy/Imaging
Thank you, Past and Present Students, Postdocs, Collaborators, Nominators, Royal Swedish Academy of Sciences, Mentors, Institutions, Family! (see Nobel Lecture for a more comprehensive list)