Illumina Sequencing Overview - Library Prep to Data Analysis

Explore an in-depth webinar on Illumina sequencing, covering the entire process from library preparation to data analysis. Gain insights into the Illumina Sequencing Workflow for Next Generation Sequencing, including library preparation constructs, cluster generation, and sequencing by synthesis. Learn about the biochemical processes automatically performed by Illumina sequencing devices and compare different chemistry versions and instrument engineering developments. Presented by Scott Westenberger, PhD, Staff Clinical Field Applications Scientist at Illumina, this 52-minute session provides a comprehensive overview applicable to various assays across different sequencing instruments. Delve into topics such as flow cell architecture, bridge amplification, dye chemistry, paired-end sequencing, and analysis overview. Moderated by Tara Namey, MS, LCGC, Senior Genomic Science Liaison at Ambry Genetics, this webinar offers valuable knowledge for professionals in molecular biology, genetics research, and clinical oncology.

Intro
Session Objectives
Illumina Sequencing Workflow
Library Prep is Critical for Successful Sequencing
What is a Cluster?
What is a flow cell?
Flow cell Architecture Random vs Pattemed
Patterned flow cells Complete control of pitch & feature size
Hybridize Fragment & Extend
Denature Double-Stranded DNA
Single-Stranded DNA
Bridge Amplification
Denature Double-Stranded Bridge
Linearization
Reverse Strand Cleavage
Read 1 Primer Hybridization
Patterned Flow Cell and ExAmp Technology
Illumina Sequencing Systems
Dye Chemistry 4-channel chemistry
2-Channel SBS Chemistry
Dye Chemistry 2-channel chemistry
Sequencing by synthesis with CMOS detection 1-channel chemistry
Illumina Chemistry Comparison
Paired-End Sequencing
Single Index Reads
Dual Index Reads
Analysis Overview