High-throughput Desorption Electrospray Ionization Mass Spectrometry for Reaction Screening, Nano-scale Synthesis and Bioanalysis

Explore high-throughput Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for reaction screening, nano-scale synthesis, and bioanalysis in this 33-minute webinar presented by Nicolás Morato, a Ph.D. candidate in Analytical Chemistry at Purdue University. Delve into the applications of DESI-MS in drug discovery workflows, focusing on its ability to generate and test large compound libraries rapidly. Learn about the Purdue Make It System and how high-throughput DESI-MS serves as a single platform for drug discovery. Examine case studies on organic reaction screening, optimization of Lomustine synthesis, and late-stage functionalization of Ezetimibe. Discover the potential of DESI-MS in assessing reactivity trends, performing online structural confirmation through MS/MS analysis, and conducting high-throughput biological assays. Gain insights into label-free DESI-MS bioassays and enzymatic assays for acetylcholinesterase and sulfotransferase 281b. Understand how this technique combines screening, nanoscale synthesis, and bioassays on a single platform, revolutionizing the speed and efficiency of drug discovery processes.

Intro
Introduction: DESI and Ambient lonization
Transition: From Imaging to High Throughput Screening
Introduction: Going Faster
Purdue Make It System: Overview
High Throughput DESI-MS: a single platform for drug discovery
High Throughput Screening: Organic Reactions
Optimization of Lomustine Synthesis
Late-stage Functionalization of Ezetimibe
Assessment of Reactivity Trends
Online Structural Confirmation: MS/MS Analysis
Screening, nanoscale synthesis and bioassays on a single platform
High Throughput Biological Assays
Label-free DESI-MS bioassays
DESI-MS Enzymatic Assays: Acetylcholinesterase
DESI-MS Enzymatic Assays: Sulfotransferase 281b