Glow in the Dark - Using Fluorescence to Observe DNA in a Living Cell

Explore the fascinating world of DNA visualization in living cells through fluorescence techniques in this 53-minute lecture by Dr. Elizabeth Hinde. Discover how physics enables real-time observation of DNA network spatial reorganization, potentially serving as an epigenetic control layer for gene expression. Learn about the challenges of optical microscopy in observing DNA organization due to diffraction limits, and understand the breakthrough collaborations between physics, chemistry, and biology that led to super-resolution imaging. Delve into the intricacies of genome architecture, live cell nanoscale genome dynamics, and how nuclear architecture facilitates DNA repair factor navigation. This lecture, part of the University of Melbourne's Science Festival, offers insights into the complex relationship between genome size, organism complexity, and gene count, while highlighting the potential functions of non-coding DNA.

Intro
LECTURE OUTLINE
DNA IS THE MOLECULE THAT ENCODES THE BLUEPRINT OF LIFE
AND YET GENOME SIZE DOES NOT PREDICT ORGANISM COMPLEXITY
AND GENOME SIZE DOES NOT PREDICT GENE COUNT (CODING DNA)
SO, WHAT FUNCTION DOES ALL OF THE NON-CODING DNA SERVE 7
DYNAMIC REARRANGEMENT IN LIVE CELL GENOME ARCHITECTURE
OPTICAL MICROSCOPY ENABLES US TO OBSERVE A LIVING CELL
BUT DIFFRACTION LIMITS OBSERVATION OF DNA ORGANISATION
SO, WHAT ASPECTS OF GENOME ARCHITECTURE CAN WE SEE ?
BREAKTHROUGH COLLABORATION-PHYSICS CHEMISTRY BIOLOGY
ADVENT OF SUPER-RESOLUTION - SEEING GENOME ARCHITECTURE
SO, HOW DO WE GET AROUND DIFFRACTION WITH FLUORESCENCE
WHAT DOES THIS MEAN FOR IMAGING GENOME ARCHITECTURET
HOW CAN WE IMAGE LIVE CELL NANOSCALE GENOME DYNAMICS
HISTONE FRET - REPORTS NUCLEAR WIDE NUCLEOSOME PROXIM..
QUESTION? HOW DOES DNA REPAIR MACHINERY SENSE DNA DAMAGE
NUCLEAR ARCHITECTURE FACILITATES REPAIR FACTOR NAVIGATIR
LECTURE SUMMARY