Development of an On-Chip Binding Assay to Select Clones Expressing Bispecific Antibodies

Explore an innovative approach to cell line development for bispecific antibodies in this 24-minute webinar presented by Dr. Shu Wang, Principal Investigator at Incyte. Learn about the challenges in creating cell lines expressing bispecific antibodies and discover a novel on-chip binding assay developed in collaboration with Bruker Cellular Analysis. Gain insights into the process of labeling ligands, performing on-chip binding assays, and analyzing fluorescence intensity to calculate stoichiometric ratios. Understand how this new method compares to traditional techniques and its potential to streamline cell line development while improving efficiency. Delve into the validation process, exploring the correlation between on-chip binding assay results and actual ratios from bulk pools, mini pools, and clones. Examine the impact of this innovative approach on fed-batch titer and product quality, and discover how it addresses the complexities of heterodimer, halfmer, and homodimer production in bispecific antibodies.

Development of an on chip binding assay to select clones expressing bispecific antibodies