Delivering Fast, Flexible Next-Generation Sequencing through Innovations in Chemistry and Engineering

Explore a webinar on next-generation sequencing innovations presented by Dr. Timothy Looney, Senior Director of Immunology and Bioinformatics at Singular Genomics. Discover how advancements in chemistry and engineering are revolutionizing DNA sequencing platforms to provide high accuracy, speed, and flexible throughput. Learn about the impact of rapid sequencing and flexible throughput on reducing turnaround time in research and clinical NGS applications. Gain insights into optical ensemble sequencing by synthesis, rapid nucleotide incorporation techniques, and high-performance computing requirements for image processing and basecalling. Examine the technical reproducibility, correlation with Illumina NextSeq™ 2000, sequencing quality metrics, and accuracy across challenging genomic features. Understand the importance of sequencing and batching in research and clinical sequencing turnaround times, and explore the variations in required turnaround for different applications.

Intro
SEQUENCING AND BATCHING ARE KEY CONTRIBUTORS TO LONG TURNAROUND FOR RESEARCH AND CLINICAL SEQUENCING
GREAT VARIATION IN REQUIRED TURNAROUND FOR CLINICAL AND RESEARCH APPLICATIONS
OPTICAL ENSEMBLE SEQUENCING BY SYNTHESIS VIA FLUORESCENTLY LABELED REVERSIBLE TERMINATED NUCLEOTIDES
RAPID NUCLEOTIDE INCORPORATION, IMAGING AND CLEAVAGE
HIGH PERFORMANCE COMPUTING REQUIRED FOR RAPID IMAGE PROCESSING, BASECALLING AND INTERPRETATION
A COMBINATION OF GPU AND CPU PROCESSING DELIVERS SPEED & VERSATILITY
HIGH TECHNICAL REPRODUCIBILITY
HIGH CORRELATION WITH ILLUMINA NEXTSEQ™ 2000
SEQUENCING QUALITY METRICS
ALIGNMENT AND COVERAGE METRICS
READ ACCURACY AND ERROR MODES
ACCURACY ACROSS CHALLENGING GENOMIC FEATURES