Bacteriological Diagnosis of Tuberculosis - Smear and Culture

Learn about the bacteriological diagnosis of tuberculosis through smear microscopy and culture techniques in this comprehensive 30-minute video. Explore conventional diagnostic methods, including sputum collection and triple packaging of infectious material. Examine the appearance of TB bacilli in smears and understand the limitations of smear microscopy. Discover advancements like Light Emitting Diode (iLED) microscopy and solid culture techniques using Lowenstein Jensen and agar media. Delve into liquid culture systems, focusing on the MGIT960 method, its components, principles, and result interpretation. Investigate species identification of non-tuberculous mycobacteria through biochemical tests and molecular techniques. Gain insights into whole genome sequencing for NTM and grasp key messages for effective TB diagnosis.

Intro
Bacteriological Diagnosis of Tuberculosis: Smear and Culture
Diagnosis of TB
Good quality sputum / gastric aspirate
Triple Packaging of Infectious Material
Conventional Bacteriological diagnostic methods for TB diagnosis
Appearance of TB bacilli in smears
Limitations of Smear Microscopy
Light Emitting Diode (iLED) microscope
Solid Culture - Egg based Lowenstein Jensen & Agar Media
Limitations of solid culture for TB diagnosis
Features of MGIT960
Components and Principle MGIT960
Identification and confirmation of M.tb growth in liquid culture
BACTEC MGIT960 - Result output
Disadvantages of MGIT 960
Species Identification of NTM Biochemical Tests
Molecular testing
Whole genome Sequencing: NTM
Key messages