Analysis of Fluorescently Labeled Contractile Subcellular Structures - Instantaneous Flow Tracking

Explore the analysis of fluorescently labeled contractile subcellular structures in this 54-minute lecture by Dr. Alexandre Matov from Cornell University. Delve into the challenges of studying cytoskeletal microtubule and F-actin meshwork dynamics using fluorescent speckle microscopy (FSM). Learn about the innovative instantaneous flow tracking (IFT) algorithm, based on graph theory, which captures complex speckle motions in time-lapse image sequences. Discover how this method applies bi-objective optimization to maximize accepted links between speckles while minimizing global linking costs. Examine the algorithm's application to FSM time-lapse series and its ability to capture rapidly changing, dense, and multi-directional speckle flows in cytoskeletal dynamics. Gain insights into Dr. Matov's research on microtubules, their role in cellular processes, and their significance as targets in oncology. Explore topics such as chromosome segregation, mitotic spindle modeling, and the analysis of circulating tumor cells. Understand the impact of chemotherapeutics on microtubule dynamics and the importance of these studies in advancing cancer research.

Intro
Chromosome Segregation
A Model of the Mitotic Spindle
Algorithm for Tracking Dense Anti-Parallel Flow
Multi-objective optimization
Local and Global Speckle Motion Models
Datasets for Software Evaluation
Benchmarking using Particle Image Velocimetry Data
Applications of Particle Flow Tracking
Monastrol-Monopole Spindles Flux
Circulating Tumor Cells: Source of Liquid Biopsy
Image Analysis of Circulating Tumor Cells
Taxanes Target and Bind to Microtubules
Microtubule Bundling (Arrows) in Result of Drug Treatment
Speckle Formation