A Spatially-Resolved Transcriptional Atlas of the Murine Dorsal Pontine Tegmentum

Explore a 40-minute webinar on creating a spatially-resolved transcriptional atlas of the murine dorsal pontine tegmentum. Delve into the complex region of the dorsal pontine tegmentum (PnTg) and its role in regulating vital functions. Learn about the application of single-nucleus RNA-seq (snRNA-seq) to identify neuronal subtypes and the use of multiplexed error robust fluorescence in situ hybridization (MERFISH) for spatial mapping. Discover an innovative dashboard developed in collaboration with HEAVY.AI that enables real-time interactive visualization and analysis of spatial-transcriptomic data using GPU and CPU hardware. Gain insights into interpreting complex spatial data and making data-driven decisions. Presented by Stefano Nardone, PhD, a postdoctoral scientist at Harvard Medical School and Beth Deaconess Medical School, specializing in statistical data analysis, next-generation sequencing, and spatial transcriptomics.

A spatially-resolved transcriptional atlas of the murine dorsal pontine tegmentum