Laboratory Diagnosis of Anaerobic Infections - Lecture 8

Explore the laboratory diagnosis of anaerobic infections in this comprehensive lecture. Delve into early infection signs, proper sample selection, transportation, and cultivation techniques. Gain insights into identification and susceptibility testing methods, and understand the pathogenesis of clinically relevant anaerobic bacteria. Learn about swabs, transport media, growth media selection, and anaerobiosis control. Discover various identification options, from simple to advanced, including commercial methods, MALDI-TOF mass spectrometry, and 16S sequencing. Examine susceptibility testing techniques such as agar dilution, microbroth dilution, and EUCAST disc diffusion methodology. Investigate the pathogenesis of gas gangrene, tetanus, botulism, and actinomycosis, enhancing your knowledge of anaerobic infections and their laboratory diagnosis.

Intro
Learning Objectives
Swabs and transport media
Growth media selection
Anaerobiosis: Biological Vs Chemical controls
Identification: options from simple to advanced!
Commercial identification methods
MALDI-TOF mass spectrometry
16S Sequencing
Agar dilution
Microbroth dilution
EUCAST: disc diffusion development
EUCAST: disc diffusion methodology
EUCAST: disc diffusion parameters
Pathogenesis of Gas Gangrene
Tetanus ('lockjaw')
Pathogenesis of Tetanus
Botulism
Actinomycosis - clinical summary