3D Cell Migration Using a Microfluidic Endothelium-on-a-Chip Model

Explore cell migration mechanisms in a 37-minute webinar featuring Dr. Kristin Bircsak's presentation on 3D cell migration using a microfluidic endothelium-on-a-chip model. Delve into the essential process of cell migration and its role in wound healing, cancer metastasis, and immune system function. Learn about the OrganoPlate technology and its applications in studying immune cell extravasation and infiltration. Discover how to visualize and quantify T cell migration, generate chemokine gradients, and induce vascular inflammation in microfluidic chips. Gain insights into donor-to-donor differences, blocking T cell extravasation, and the compatibility of immune migration assays with various cell types. Understand the potential of human tissue models for developing better therapies and advancing our understanding of complex biological phenomena.

Intro
Human Tissue Models for better therapies
Meet the OrganoPlate
OrganoPlate® family
Immune cell migration
Need of novel in vitro immune migration models
Based on publication
Formation of HMEC-1 vessels in the OrganoPlate 3-lane
HMEC-1 vessels can be maintained in immune cell medium
Visualization and quantification of T cells in microfluidic chip
Perfusion of HMEC-1 vessels with fluorescently labelled T cells
ET cells and vasculature interplay
Generation of chemokine gradients in the OrganoPlate 3-lane
T cell extravasation in the presence of chemokine gradients
Donor-to-donor differences are preserved
Induction of vascular inflammation Upregulation of adhesion molecules
Blocking extravasation of T cells
Immune infiltration in the tumor compartment
Immune migration assays are also compatible with HUVECS
The OrganoPlate as a tool to study immune cell extravasation and infiltration
Cell migration in the OrganoPlate